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KMID : 0903519860290020175
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
1986 Volume.29 No. 2 p.175 ~ p.181
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Dissimilatory Nitrate Reduction Characteristics of Indigenous Soybean Rhizobia Distributed in Korean Soils
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이상규/Lee, Sang Kyu
최영주/조무제/최용락/유진창/윤한대/Choi, Young Ju/Cho, Moo Je/Choi, Yong Lark/Ryu, Jin Chang/Yun, Han Dae
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Abstract
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Eightyseven strains of indigenous Rhizobia were isolated from the nodule of soybean cultivar, Danyup, cultivated in four different soils sampled from continuously soybean cultivated and newly reclaimed fields. The strains were grouped into Bradyrhizobium japanicum (slow-grower: 55 strains) and Rhizobium fredii (fast-grower: 32 strains). The both groups could be divided into two sub-groups according to the denitrification characteristics, that is, denitrifying fast-grower (F-Ⅰ), nitrate respiring fast-grower (F-Ⅱ), denitrifying slow-grower (S-Ⅰ). and nitrate respiring slow-grower (S-Ⅱ). Among the 87 isolates, F-Ⅰ, F-Ⅱ, S-Ⅰ and S-Ⅱ sub-groups were 10, 22, 48 and 7 strains, respectively. The one-and two-dimensional polyacrylamide gel electrophoretic pattern of the four sub-groups were compared and discernible difference was observed between fast and slow-grower, but the difference was not discernible between subgroups within the same growth rate group.
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